预期应用
ELISA法定量测定人血清、血浆、尿液或其它相关液体中本周蛋白(Bence-Jones protein)含量。
介绍
本周蛋白（Bence-Jones protein）是多发性骨髓瘤的典型标志物，或称其为“免于球蛋白轻链”标志物。早在1845年由一位内科医生兼化学病理学家Henry Bence Jones首次描述了这种蛋白，它可被氨基水杨酸、三氯醋酸、硝酸和盐酸沉淀，加热到45-60℃时，沉淀又再现，故又名为凝溶蛋白。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中本周蛋白水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入本周蛋白、生物素化的抗人本周蛋白抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的本周蛋白呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human Bence-Jonesprotein,BJP concentrations in cell culture supernates, serum, urine, and plasma.
Introduction
A Bence Jones protein is a monoclonal globulin protein found in the blood or urine. The isolated finding of a Bence Jones protein is known as monoclonal gammopathy of uncertain significance. Finding this protein in the context of end-organ manifestations such as renal failure, lytic bone disease, or anemia, or large numbers of plasma cells in the bone marrow of patients can be diagnostic of multiple myeloma.
The proteins are antibody immunoglobulin free light chains (paraproteins) and are produced by neoplastic plasma cells. The light chains can be detected by heating or electrophoresis of concentrated urine. Light chains precipitate when heated to 50 - 60 degrees C and redisolve at 90 -100 degrees C. These tests are essential in patients suspected of having Bence Jones proteins in their urine as these proteins dont react with the reagents normally utilized in urinalysis dipsticks. This leads to false negative results in people with Bence Jones proteins in their urine undergoing standard urinalysis. There are various rarer conditions which can produce Bence Jones proteins, such as Waldenstroms macroglobulinemia and other malignanices.
Bence-Jones proteins are a rare finding in urine, but if present, they are usually associated with:
multiple myeloma, Waldenstroms macroglobulinaemia ,chronic lymphocytic leukaemia - when your body produces abnormal white blood cells, which then affect your immune system; amyloidosis.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for Bence-Jones protein has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Bence-Jones protein present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for Bence-Jones protein is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Bence-Jones protein bound in the initial step. The color development is stopped and the intensity of the color is measured. | 
