ELISA法定量测定小鼠血清、血浆或其它相关液体中HP IgG含量。

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中HP IgG水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入HP抗原、生物素化的抗小鼠HP IgG抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的HP IgG呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

This immunoassay kit allows for the specific measurement of mouse Helicobacter Pylori (HP) IgG concentrations in serum and plasma.

 

H. pylori strains are classified into two broad groups - those that express both VacA and CagA (type I) and those that produce neither (type II). Type I strains are predominate in patients with ulcers and cancer. Up to 50% of adults is infected with H. pylori, but most of them are asymptotic and will not develop ulcer. The reason is they are infected with type II. 80-100% of patients with duodenal ulcer disease produce CagA antibodies against a 128 kd antigen compared with 60-63% of H. pylori-infected persons with gastritis only, indicating that serologic responses to the 128 kd protein are more prevalent among H. pylori-infected persons with duodenal ulcers than infected persons without peptic ulceration. In H. pylori-infected patients who develop gastric cancer, serum IgG against CagA 94% sensitive and 93% specific, indicating that detection of antibodies to CagA is useful marker for diagnosis of duodenal ulcer and gastric cancer.

 

This assay employs the quantitative sandwich enzyme immunoassay technique. Partially purified H. pylori antigens are immobilized on the wells of a microwell plate. Diluted sample is added to the wells. IgG antibodies specific to H. pylori, if present, bind to the antigen on the microwells. Excess IgG antibodies are washed away with buffer.  Anti-Mouse IgG Enzyme Conjugate is added to the wells. The conjugate binds with the antigen-antibody complex, if present, on the plate. Excess enzyme conjugate is washed away and a color is developed by the addition of an enzyme substrate. The intensity of the color corresponds directly to the amount of antibody present. The color intensity read on the spectrophotometer is a direct interpretation of H. pylori specific antibodies in the sample.

This immunoassay kit allows for the specific measurement of mouse Helicobacter Pylori (HP) IgG concentrations in serum and plasma.

 

H. pylori strains are classified into two broad groups - those that express both VacA and CagA (type I) and those that produce neither (type II). Type I strains are predominate in patients with ulcers and cancer. Up to 50% of adults is infected with H. pylori, but most of them are asymptotic and will not develop ulcer. The reason is they are infected with type II. 80-100% of patients with duodenal ulcer disease produce CagA antibodies against a 128 kd antigen compared with 60-63% of H. pylori-infected persons with gastritis only, indicating that serologic responses to the 128 kd protein are more prevalent among H. pylori-infected persons with duodenal ulcers than infected persons without peptic ulceration. In H. pylori-infected patients who develop gastric cancer, serum IgG against CagA 94% sensitive and 93% specific, indicating that detection of antibodies to CagA is useful marker for diagnosis of duodenal ulcer and gastric cancer.

 

This assay employs the quantitative sandwich enzyme immunoassay technique. Partially purified H. pylori antigens are immobilized on the wells of a microwell plate. Diluted sample is added to the wells. IgG antibodies specific to H. pylori, if present, bind to the antigen on the microwells. Excess IgG antibodies are washed away with buffer.  Anti-Mouse IgG Enzyme Conjugate is added to the wells. The conjugate binds with the antigen-antibody complex, if present, on the plate. Excess enzyme conjugate is washed away and a color is developed by the addition of an enzyme substrate. The intensity of the color corresponds directly to the amount of antibody present. The color intensity read on the spectrophotometer is a direct interpretation of H. pylori specific antibodies in the sample.