预期应用
ELISA法定量测定人血清、血浆或其它相关液体中RBP4含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中RBP4水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入RBP4抗原、生物素化的抗人RBP4抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的RBP4呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human RBP4 concentrations in serum and plasma.
Introduction
Retinol binding protein (RBP) 4 is the only specific transport protein for vitamin A in the circulation whose function is to deliver vitamin to target tissues. In obesity and type 2 diabetes, expression of Glut4 is significantly impaired in adipocytes. Glucose transport via Glut4 is the rate-limiting step for glucose use by muscle and adipose tissue. Yang et al. noted that adipocytespecific deletion of Gluts led to notable elevation of RBP4 causing systemic insulin resistance, and that reduction of RBP4 improved insulin resistance. This identified a novel role of RBP4 in regulating insulin action and RBP4 is recorded as an adipocytederived hormone. Thus, measurement of serum or plasma RBP4 is a useful means for understanding of metabolic disorders.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for RBP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RBP4 present is bound by the immobilized antibody. An
enzyme-linked monoclonal antibody specific for RBP4 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells
and color develops in proportion to the amount of RBP4 bound in the initial step. The color
development is stopped and the intensity of the color is measured. | 
