预期应用
ELISA法定量测定兔子血清、血浆或其它相关液体中8-异构前列腺素（8-epi PGF2α）含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中PGF2α水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入PGF2α、生物素化的抗兔子PGF2α抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的PGF2α呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Rabbit 8-Isoprostane concentrations in cell culture supernates, serum and plasma.
Introduction
The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. Isoprostanes appear as artifacts in tissue and plasma samples which have undergone oxidative degradation during prolonged or improper storage. They also appear in the plasma and urine under normal conditions and are elevated by oxidative stress. At least one of the isoprostanes, 8-isoprostane (8-epi PGF2α), has been shown to have biological activity. It is a potent pulmonary and renal vasoconstrictor1 and has been implicated as a causative mediator of hepatorenal syndrome and pulmonary oxygen toxicity.2 8-Isoprostane has been proposed as a marker of antioxidant deficiency and oxidative stress and elevated levels have been found in heavy smokers.3 8-Isoprostane levels are also a relative indicator of sample integrity for lipid-containing samples such as serum, plasma, and whole cell preparations.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for 8-Isoprostane has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any 8-Isoprostane present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for 8-Isoprostane is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 8-Isoprostane bound in the initial step. The color development is stopped and the intensity of the color is measured. | 
