ELISA法定量测定人血清、血浆或其它相关液体中葡萄糖6磷酸异构酶(GPI)含量。

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中GPI水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入GPI、生物素化的抗人GPI抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的GPI呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

This immunoassay kit allows for the specific measurement of human glucose-6-phosphate isomerase,GPI concentrations in cell culture supernates, serum, and plasma.

Glucose-6-phosphate isomerase (GPI) has an essential function in both catabolic glycolysis and anabolic gluconeogenesis and is universally distributed among Eukaryotes, Bacteria, and some Archaea.GPI is expressed by the corpus luteum on days 6–9 of pregnancy, the time at which implantation-promoting activity has been found in corpora lutea. Passive immunization against GPI reduced the number of implantation sites in pregnant ferrets in a dose-dependent manner. GPI is a multifunctional protein. Although first identified for its role in glycolysis, GPI has since been implicated in neural growth, lymphocyte maturation, and metastasis. This study demonstrates a previously uncharacterized function of this protein that may represent the natural motility-stimulating activity that has been co-opted by tumor cells.Glucose 6-phosphate (also known as Robison ester) is glucose sugar phosphorylated on carbon 6. This compound is very common in cells as the vast majority of glucose entering a cell will become phosphorylated in this way.

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal

antibody specific for GPI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPI present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for GPI is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPI bound in the initial step. The color development is stopped and the intensity of the color is measured.