ELISA法定量测定鸭血清、细胞培养物上清或其它相关液体中IL-1含量。

 

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中IL-1水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入IL-1抗原、生物素化的抗鸭IL-1抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的IL-1呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

This immunoassay kit allows for the specific measurement of total duck IL-1 concentrations in cell culture supernates, serum, and plasma.

IL-1α and β are 17 kDa polypeptides that share 25% identity at the amino acid level. Although the product of distinct genes, both IL-1α and β bind to the same receptors, generally producing similar responses. Cells known to express IL-1 include osteoblasts, macrophages, fibroblasts, keratinocytes and glial cells. The diverse functions attributed to IL-1 include T cell activation with subsequent IL-2 secretion and IL-2 receptor expression, increased synthesis of acute phase proteins by hepatocytes, and regulation of bone metabolism in developing bone. Receptors for IL-1 are of two types: the first (or IL-1 R type I) is an 80 kDa signal transducing molecule found on T cells, fibroblasts, hepatocytes and keratinocytes; the second (or IL-1 R type II) is a “decoy” 68 kDa molecule found on neutrophils and B cells. It does not transduce a signal upon IL-1 binding. In general, IL-1α binds better to the type I receptor. The presence of either the type I or type II receptor, coupled with the cell type and relative cell surface receptor density can provide information on the activation state cells and their ability to respond to selective growth factors.

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for IL-1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.