本试剂盒应用双抗体夹心酶标免疫分析法测定标本中HGF水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入HGF抗原、生物素化的抗人HGF抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的HGF呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human HGF concentrations in serum and plasma.
Introduction
Hepatopoietin-A/hepatocyte growth factor (HPTA,HGF) is a heparin-binding polypeptide growth factor which consists of a heavy and a light polypeptide chain with molecular weights of 70,000 and 35,000, respectively. It is a mitogen for primary hepatocytes and may mediate regeneration after liver damage. In the liver, HGF has been shown to stimulate proliferation and morphogenesis of biliary epithelial cells in addition to its well-known effects on hepatocytes.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for HGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HGF present is bound by the immobilized antibody. An
enzyme-linked monoclonal antibody specific for HGF is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells
and color develops in proportion to the amount of HGF bound in the initial step. The color
development is stopped and the intensity of the color is measured.
