预期应用
ELISA法定量测定小鼠血清、细胞培养物上清或其它相关液体中白介素2（IL-2）含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中IL-2水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入IL-2抗原、生物素化的抗小鼠IL-2抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的IL-2呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
This immunoassay kit allows for the specific measurement of total Mouse Interleukin 2，IL-2 concentrations in cell culture supernates, serum, and plasma.
Introduction
Interleukin 2 (IL-2) is a pleiotropic cytokine produced primarily by mitogen- or antigen-activated
T lymphocytes . IL-2 plays a key role in promoting the clonal expansion of antigen-specific T cells. In addition, IL-2 has also been shown to mediate multiple immune responses on a variety of cell types. IL-2 stimulates the proliferation of thymocytes; stimulates the proliferation and differentiation of activated B cells; promotes the growth, differentiation and cytocidal activity of monocytes; induces the growth of and cytokine production and cytolytic activity by natural killer cells; enhances the production of lymphocyte-activated killer (LAK) cells; and induces the proliferation and differentiation of oligodendrocytes .
Interleukin 2，IL-2 is a protein of 133 amino acids (15.4 kDa). It is produced mainly by T-cells expressing the surface antigen CD4 following cell activation by mitogens or allogens under physiological conditions. IL-2 displays significant anti-tumor activity for a variety of tumor cell types since it supports the proliferation and clonal expansion of T-cells that specifically attack certain tumor types.
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-2 present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for IL-2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
| 
