预期应用
ELISA法定量测定人血清、血浆、细胞培养物上清或其它相关液体中NGF含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中NGF水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入NGF抗原、生物素化的抗人NGF抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的NGF呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度
Intended use
This immunoassay kit allows for the specific measurement of human NGF concentrations in cell culture supernates, serum, and plasma.
Introduction
Nerve growth factor (NGF), a naturally occurring molecule in the body which stimulates the growth and differentiation of the sympathetic and certain sensory nerves. NGF is a protein that consists of 3 types of polypeptide chains -- alpha, beta and gamma -- that interact to form the protein. The NGF beta chain (NGFB) is solely responsible for the nerve growth stimulating activity of NGF. The NGFB gene is in chromosome band 1p22. An ointment containing NGF has been reported to help heal bedsores (decubiti).
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for NGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NGF present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for NGF is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
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