预期应用
ELISA法定量测定猴血清、血浆、或其它相关液体中FSH含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中FSH水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入FSH抗原、生物素化的抗猴FSH抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的FSH呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of monkey FSH concentrations in serum and plasma.
Introduction
Follicle-Stimulating Hormone (FSH) and Luteinizing Hormone (LH) are intimately involved in the control of the growth and reproductive activities of the gonadal tissues. The levels of circulating FSH and LH are controlled by these sex hormones through a negative feedback relationship. FSH is a glycoprotein secreted by the basophil cells of the anterior pituitary. Gonadotropin-releasing hormone (GnRH), produced in the hypothalamus, controls the release of FSH from the anterior pituitary. Like other glycoproteins, such as LH, TSH, and HCG, FSH consists of subunits designated as alpha and beta. Hormones of this type have alpha subunits that are very similar in structure; therefore the biological and immunological properties of each are dependent on the unique beta subunit.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for FSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FSH present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for FSH is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSH bound in the initial step. The color development is stopped and the intensity of the color is measured.
| 
