本试剂盒应用双抗体夹心酶标免疫分析法测定标本中sCD30L水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入sCD30L抗原、生物素化的抗人sCD30L抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的sCD30L呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human sCD30L concentrations in cell culture supernates, serum, and plasma.
Introduction
The human CD30 ligand, CD153, is a 40kDa Type II transmembrane glycoprotein. It interacts with its receptor, CD30, as a homotrimer Soluble trimeric CD153 binds to membrane-anchored CD30 with a relatively high affinity and is very effective in triggering cell death and TNFα. CD153 is a member of the TNF superfamily which is primarily expressed by activated T cells, B cells and monocytes. Its function in programmed cell death has been proven, as well as its involvement in disorders like a variety of lymphoid malignancies like Hodgkin’s disease and anaplastic large cell lymphoma.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for sCD30L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD30L present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for sCD30L is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD30L bound in the initial step. The color development is stopped and the intensity of the color is measured.
