本试剂盒应用双抗体夹心酶标免疫分析法测定标本中PAI水平。用纯化的抗体包被微孔板，制成固相抗体，往包被抗体的微孔中依次加入PAI、生物素化的抗PAI抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的PAI呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Rabbit Plasminogen activator inhibitor ,PAI concentrations in plasma.
Introduction
Plasminogen activator inhibitor (PAI) is serpin family member that inhibits tissue- and urokinase-type plaminogen activators (t-PA, u-PA). This protein appears to be an important regulator of plasminogen activation by t-PA and extracellular proteolysis by u-PA . The plasminogen activator proteolytic enzyme systems are important not only for fibrinolysis but also for extracellular matrix remodeling, and have been implicated in a number of normal and pathological processes including angiogenesis, ovulation and embryogenesis, thrombotic and hemorrhagic disorders, connective tissue diseases, neoplasm and sepsis. PAI-1 is a prognosticator in breast cancer, gastric cancer, various forms of lung cancer and cervical cancer.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for PAI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PAI present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for PAI is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAI bound in the initial step. The color development is stopped and the intensity of the color is measured.
