Intended use
This immunoassay kit allows for the specific measurement of total progesterone concentrations of natural and recombinant rat progesterone in plasma and serum.
Introduction
Progesterone is a steroid hormone which plays an important role in the preparation for and maintenance of pregnancy. It is synthesized from cholesterol via pregnenolone, then rapidly metabolized to pregnanediol, for the most part, in the liver. The ovary and placenta are the major production sites; but a small amount is also synthesized by the adrenal cortex in both men and women. Circulating progesterone levels, which are characteristically low during the follicular phase, increase sharply during the luteal phase of menstrual cycles, reaching a maximum some 5 to 10 days after the midcycle LH peak. Unless pregnancy occurs, a steep decline to follicular levels sets in about 4 days before the next menstrual period.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for progesterone has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled progesterone and biotin unlabeled antigens (Standards or samples) with the pre-coated antibody specific for progesterone. The more the amount of PROGESTERONE in samples, the less the biotin labeled progesterone bound by pre-coated antibody. Vice versa. Then HRP labeld avidin and substrate solution are added to the wells, respectively. And the color develops in opposite to the amount of PROGESTERONE bound in the initial step. The color development is stopped and the intensity of the color is measured.
