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预期应用
ELISA法定量测定犬血清、血浆、或其它相关液体中孕激素含量。
概述
孕激素主要由黄体产生，故又叫黄体酮。孕激素通常要在孕激素作用的基础上才能发挥其作用。孕激素的主要作用，是促进子宫内膜特别是腺体的增长，为接纳受精卵做好准备，如受精卵着床后，使胚胎继续发育。对子宫内膜癌及增生过度的内膜腺体，孕激素则能使之萎缩退化。此外还能抑制子宫收缩，并减弱子宫对催产素的敏感性，使子宫活动减少，而起保胎作用。同时还与孕激素一起使乳腺发育，为产乳作好准备。
实验原理
本试剂盒应用竞争抑制酶标免疫分析法测定标本中孕激素水平。用纯化的抗体包被微孔板，制成固相抗体，固相抗体与一定量的生物素标记的孕激素和非标记抗原(标本或标准品)进行竞争抑制反应，待测定的标本量越多，抗体与生物素标记的孕激素结合就越少，反之结合就多。最后再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的孕激素呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of total progesterone concentrations of natural and recombinant canine progesterone in plasma and serum.
Introduction
Progesterone is a steroid hormone which plays an important role in the preparation for and maintenance of pregnancy. It is synthesized from cholesterol via pregnenolone, then rapidly metabolized to pregnanediol, for the most part, in the liver. The ovary and placenta are the major production sites; but a small amount is also synthesized by the adrenal cortex in both men and women. Circulating progesterone levels, which are characteristically low during the follicular phase, increase sharply during the luteal phase of menstrual cycles, reaching a maximum some 5 to 10 days after the midcycle LH peak. Unless pregnancy occurs, a steep decline to follicular levels sets in about 4 days before the next menstrual period.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for progesterone has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled progesterone and biotin unlabeled antigens (Standards or samples) with the pre-coated antibody specific for progesterone. The more the amount of progesterone in samples, the less the biotin labeled progesterone bound by pre-coated antibody. Vice versa. Then HRP labeld avidin and substrate solution are added to the wells, respectively. And the color develops in opposite to the amount of progesterone bound in the initial step. The color development is stopped and the intensity of the color is measured. | 
