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产品名称：人血管内皮细胞粘附分子1(VCAM-1/CD106)ELISA kit  | | 英文名称：Human Vascuolar cell adhesion molecule 1,VCAM-1 ELISA kit | | 产品分类：人CD和AM类酶联免疫吸附测定试剂盒[ELISA Kit for Human CD & AM] | | 产品编号：E0142h | | 检验方法：ELISA | | 包 装：96T | | 价 格：3680 | | 品 牌：Uscnlife | | 说明书下载："中文下载""Instruction" | | 产品说明：人血管内皮细胞粘附分子1(VCAM-1/CD106)ELISA kit 概述 |
- 预期应用
- ELISA法定量测定大鼠血清、细胞培养物上清或其它相关液体中sVCAM-1含量。
- 概述
- VCAM-1是一种表达在淋巴细胞、单核细胞等细胞表面的一种膜蛋白。其基因编码产物相对分子质量为110×103，分子的细胞外部分由6个Ig样结构域组成。VCAM-1的受体是极迟抗原-4(VLA-4)和整合素a4 B7，均属于整合素家族。VCAM-1表达在大多数单个核细胞上，包括嗜酸细胞、淋巴细胞、单核细胞和嗜碱细胞上，但不表达于中性粒细胞上，因此，VCAM-1可选择性促进单个核细胞的黏附。VCAM-1的这一特性可能在恶性血液病骨髓浸润、髓外转移中起重要作用。
- 实验原理
- 本试剂盒应用双抗体夹心酶标免疫分析法测定标本中sVCAM-1水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入sVCAM-1抗原、生物素化的抗大鼠sVCAM-1抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的sVCAM-1呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
- Intended use
- This immunoassay kit allows for the specific measurement of total rat soluble Vascular Cell Adhesion Molecule-1 (sVCAM-1) concentrations in cell culture supernates, serum, and plasma.
- Introduction
- VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1B, IL-4, TNF-a and IFN-y. VCAM-1 binds to leukocyte integrins VLA-4 and a4 B7. The rat and mouse VCAM-1 proteins share approximately 76% amino acid similarity.
- During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/a B7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation.
- Test principle
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for sVCAM-1 has been pre-coated onto a microplate. Standards and samples are
- pipetted into the wells and any sVCAM-1 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for sVCAM-1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sVCAM-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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