预期应用
ELISA法定量测定大鼠血清、血浆、细胞培养物上清或其它相关液体中ASAb IgG含量。
概述
精子是一种免疫抗原，可产生自家抗精子抗体，继而可在女方血液中或生殖道中产生抗体而造成不孕，同时对精卵融合具有抑制作用。抗精子抗体有二种主要类型，一种是使精子凝结的抗体，一种是使精子制动的抗体，在精液和血浆中可测出抗体。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中AsAb水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入AsAb抗原、生物素化的抗人AsAb抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的AsAB呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rat AsAb IgG concentrations in cell culture supernates, serum, and plasma.
Introduction
Antisperm antibodies (anti-spermatozoa antibodies, sperm antibodies) in the form of autoantibodies in male and alloantibodies in female patients directed against sperm antigens may prevent fertilization of the oocyte into the female genital tract and are therefore one of the major reasons for an immunologically induced infertility. Fertility disorders of unknown etiology of male as well as female patients result to a considerably amount (up to 20%) from antisperm antibodies.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for AsAB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AsAB present is bound by the immobilized antibody. An
enzyme-linked monoclonal antibody specific for AsAB is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells
and color develops in proportion to the amount of AsAB bound in the initial step. The color
development is stopped and the intensity of the color is measured. | 
