预期应用
ELISA法定量测定大鼠血清、血浆、细胞培养物上清或其它相关液体中DHT含量。
概述
睾酮在其靶器官（如附睾和前列腺）内，被5a-还原酶还为双氢睾酮，再与靶细胞内的受体结合而发挥作用, DHT可诱导男性外生殖系统诸如阴囊和阴茎的发育。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中DHT水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入DHT抗原、生物素化的抗大鼠DHT抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的DHT呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rat DHT concentrations in cell culture supernates, serum, and plasma.
Introduction
5-a-Dihydrotestosterone (DHT) is a C19 androgenic steroid hormone similar to testosterone and androstenedione. The bulk of the androgen production takes place mainly in the Leydig cells of testes. Androgens circulate in the blood bound to proteins, especially Sex hormone binding globulin (SHBG) and albumin. A trace amount of these steroid in the unbound form in the blood and are referred to as the free fractions. DHT has at least times the binding affinity for SHBG than testosterone. In males about 70% of DHT is derived from peripheral conversion of testosterone. However, in females most of the DHT is derived from the androstenedione.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for DHT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DHT present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for DHT is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHT bound in the initial step. The color development is stopped and the intensity of the color is measured.
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