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预期应用
ELISA法定量测定人血清、血浆、细胞培养物上清或其它相关液体中维生素A含量。
概述
维生素A 为机体许多重要的生理过程所必需, 如维持上皮组织的完整性, 促进机体生长发育和暗适应等
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中维生素A水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入维生素A、生物素化的抗人维生素A抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的维生素A呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human Vitamin A concentrations in cell culture supernates, serum, and plasma.
Introduction
Vitamin A is an essential human nutrient. It may be found in any of these forms: retinol, the animal form of vitamin A, is a yellow fat-soluble, antioxidant vitamin with importance in vision and bone growth, Vitamin A is a family of compounds that play an important role in vision, bone growth, reproduction, cell division, Vitamin A is important for healthy bones.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Vitamin A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Vitamin A present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Vitamin A is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VITAMIN A bound in the initial step. The color development is stopped and the intensity of the color is measured.
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