预期应用
ELISA法定量测定大鼠血清、血浆、细胞培养物上清或其它相关液体中Vitamin D含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中Vitamin D水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入Vitamin D、生物素化的抗大鼠Vitamin D抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Vitamin D呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Rat Vitamin D concentrations in cell culture supernates, serum, and plasma.
Introduction
Vitamin D is a group of fat-soluble prohormones, the two major forms of which are vitamin D2 (or ergocalciferol) and vitamin D3 (or cholecalciferol). The term vitamin D also refers to metabolites and other analogues of these substances. Vitamin D3 is produced in skin exposed to sunlight, specifically ultraviolet B radiation.
Vitamin D plays an important role in the maintenance of organ systems.Vitamin D regulates the calcium and phosphorus levels in the blood by promoting their absorption from food in the intestines, and by promoting re-absorption of calcium in the kidneys. It promotes bone formation and mineralization and is essential in the development of an intact and strong skeleton. It inhibits parathyroid hormone secretion from the parathyroid gland. Vitamin D affects the immune system by promoting immunosuppression, phagocytosis, and anti-tumor activity. Vitamin D deficiency can result from inadequate intake coupled with inadequate sunlight exposure, disorders that limit its absorption, conditions that impair conversion of vitamin D into active metabolites, such as liver or kidney disorders, or, rarely, by a number of hereditary disorders.Deficiency results in impaired bone mineralization, and leads to bone softening diseases, rickets in children and osteomalacia in adults, and possibly contributes to osteoporosis. Vitamin D deficiency may also be linked to many forms of cancer.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Vitamin D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Vitamin D present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for Vitamin D is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Vitamin D bound in the initial step. The color development is stopped and the intensity of the color is measured.
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