预期应用
ELISA法定量测定人血浆、细胞培养物上清或其它相关液体中TF含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中TF水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入TF抗原、生物素化的抗人TF抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的TF呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human TF concentrations in plasma and cell culture supernates.
Introduction
Tissue Factor is an integral membrane-bound glycoprotein (47 kD) on SDS-PAGE that requires the presence of specific phospholipids to function. It serves as both the receptor and essential cofactor(s) for VII and VIIa in initiating cell surface procoagulant activity (PCA). Once Tissue Factor was understood to initiate the extrinsic pathway of coagulation (directly activating VII that in turn activates factor X). Now, it is also known to activate factor X through the intrinsic pathway by activating factor IX which in turn activates factor X.3 The bimolecular complex of TF and factor(s) VII or VIIa activates factors IX and X by limited proteolysis, leading ultimately to thrombin generation and fibrin formation. As a potent initiator of coagulation, TF is believed to have a critical function in hemostasis and thrombogenesis. Tissue Factor, while predominantly found in lung, brain, placenta and some neoplastic tissues (e.g. breast carcinoma, melanoma), a recent investigation has shown increased Tissue Factor levels in patients diagnosed with malignant solid tumor diseases.
Test principle This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TF present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for TF is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TF bound in the initial step. The color development is stopped and the intensity of the color is measured.
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