预期应用
概述
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中FDP水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入FDP、生物素化的抗人FDP抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的FDP呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human fibrinogen degradation products (FDP) concentrations in plasma.
Introduction
Under normal conditions, the fibrinolytic process is localized on the fibrin clots because alpha2-antiplasmin and the plasminogen activator inhibitors prevent fibrinolysis from spreading. During disseminated intravascular coagulation (DIC), fibrinolysis spreads and becomes systemic, in which case the degradation of circulating fibrinogen will occur. Fragments that occur are very heterogeneous: products derived from fibrin, soluble completes, degradation products from fibrinogen, and from nonstabilized fibrin. An abnormal fibrinolytic and/or fibrinogenolytic activity shown by high levels of FDP in plasma can also be found in clinical states, such as eclampsia, carcinoma (promyelocytic leukemia), postoperative complications, cardiac and renal disorders, hepatic disorders, fibrinolysis, pulmonary embolism, and deep vein thrombosis (DVT).
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibodies specific for FDP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FDP present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for FDP is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FDP bound in the initial step. The color development is stopped and the intensity of the color is measured.
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