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预期应用
ELISA法定量测定小鼠血清、血浆、细胞培养物上清或其它相关液体中PAI-1含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中PAI-1水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入PAI-1抗原、生物素化的抗小鼠PAI-1抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的PAI-1呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of mouse PAI-1 concentrations in cell culture supernates, serum, and plasma.
Introduction
Type I plasminogen activator inhibitor (PAI-1) is a 43 kDa serpin family member that inhibits tissue- and urokinase-type plaminogen activators (t-PA, u-PA). This protein appears to be an important regulator of plasminogen activation by t-PA and extracellular proteolysis by u-PA . The plasminogen activator proteolytic enzyme systems are important not only for fibrinolysis but also for extracellular matrix remodeling, and have been implicated in a number of normal and pathological processes including angiogenesis, ovulation and embryogenesis, thrombotic and hemorrhagic disorders, connective tissue diseases, neoplasm and sepsis. PAI-1 is a prognosticator in breast cancer, gastric cancer, various forms of lung cancer and cervical cancer.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for PAI-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PAI-1 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for PAI-1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAI-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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