预期应用
ELISA法定量测定大鼠血清、血浆、细胞培养物上清或其它相关液体中IgM含量。
概述
免疫球蛋白是一组具有抗体活性的蛋白质，存在与血液、体液、外分泌液及某些细胞的膜上。本篇内除特别指出外，均为血清的免疫球蛋白测定。目前，将免疫球蛋白分为IgG、IgA、IgM、IgD、IgE等五类。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中IgM水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入IgM、生物素化的抗大鼠IgM抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的IgM呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rat IgM concentrations in cell culture supernates, serum, and plasma.
Introduction
Immunoglobulins belong to a large group of related glyco-proteins that make up approximately 20% of the serum proteins. The serum immunoglobulins react with antigens and confer immunity to individuals. All immunoglobulins share the basic structure of: 2 identical heavy chains joined by disulfide bonds to 2 identical light chains. Both the heavy (H) chains and the light (L) chains are divided into constant and variable regions. The constant regions have similar amino acid composition between all the immunoglobulin classes while the variable regions encompasses about 110 amino acids characterized by a high degree or sequence variability. Its H-chain type, based on the amino acid sequence, determines the classes of an immunoglobulin. There are 5 types of H-chains that correspond to the following immunoglobulin classes: IgG, IgA, IgM, IgD, and IgE. IgG is further subdivided into 4 subclasses with ~95% homology. There are 2 subclasses of IgA. IgG and IgA exists in serum as a monomer consisting of a single 4-polypeptide unit. IgM exists in serum as a pen tamer. IgA may also polymerize to form polymers containing 2-5 structural units. It is important to measure the level of immunoglobulins in serum for Antibody deficiency conditions, such as Primary hypogammagobulinaemia, or other immune deficiency diseases such as AIDS. When evaluating patients with recurrent infections, suspected immunodeficiency, allergic disease and many other conditions, it may be necessary to quantify the levels of immunoglobulins
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IgM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgM present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for IgM is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IgM bound in the initial step. The color development is stopped and the intensity of the color is measured.
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