本试剂盒应用双抗体夹心酶标免疫分析法测定标本中CCK水平。用纯化的抗体包被微孔板，制成固相抗体，往包被抗体的微孔中依次加入CCK、生物素化的抗大鼠CCK抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的CCK呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Rat Cholecystokinin,CCK concentrations in serum, and plasma.
Introduction
Cholecystokinin,CCK is a peptide hormone of the gastrointestinal system responsible for stimulating the digestion of fat and protein. It is the principle stimulus for delivery of pancreatic enzymes and bile into the small intestine. Cholecystokinin, previously called pancreozymin, is synthesised by I-cells and secreted in the duodenum, the first segment of the small intestine, and causes the release of digestive enzymes and bile from the pancreas and gallbladder, respectively. It also acts as a hunger suppressant. Recent evidence has suggested that it also plays a major role in inducing drug tolerance to opioids like morphine and heroin, and is partly implicated in experiences of pain hypersensitivity during opioid withdrawal.
Cholecystokinin (CCK) plays a key role in facilitating digestion within the small intestine. It is secreted from mucosal epithelial cells in the first segment of the small intestine (duodenum), and stimulates delivery into the small intestine of digestive enzymes from the pancreas and bile from the gallbladder. Cholecystokinin is also produced by neurons in the enteric nervous system, and is widely and abundantly distributed in the brain. Cholecystokinin and gastrin are highly similar peptides. Like gastrin, cholecystokinin is a linear peptide that is synthesized as a preprohormone, then proteolytically cleaved to generate a family of peptides having the same carboxy ends.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Cholecystokinin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Cholecystokinin present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Cholecystokinin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Cholecystokinin bound in the initial step. The color development is stopped and the intensity of the color is measured.
