本试剂盒应用双抗体夹心酶标免疫分析法测定标本中GLP-1水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入GLP-1抗原、生物素化的抗大鼠GLP-1抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的GLP-1呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rat GLP-1 concentrations in cell culture supernates, serum, and plasma.
Introduction
Glucagon Like Peptide-1 (GLP-1) is a peptide hormone from the intestinal mucosa, which is produced from its precursor, proglucagon by post transnational processing. The mammalian proglucagon is synthesized in the neuroendocrine L-cell of the intestine and the alpha-cells of the pancreas. It contains within its structure the sequences of glucagon and two glucagon-like peptides (GLP-1 and GLP-2) in tandem flanked at their amino and carboxyl termini by dibasic residues. GLP-1 is a 37 amino acids peptide and produced in the small intestine and in the pancreas in the rat, in either C-terminal-amidated on glycine-extended form. GLP1 (7-36) amide and its receptor are present in several brain regions and may play a role in the physiological control of feeding. Several reports have been presented as follows as to the biological activities of GLP-1. GLP-1 (7-37) and (7-36) amide is known as one of the most potent insulin secretagogues.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GLP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GLP-1 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for GLP-1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
