预期应用
ELISA法定量测定大鼠血清、血浆、细胞培养物上清或其它相关液体中BMP-7含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中BMP-7水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入BMP-7抗原、生物素化的抗大鼠BMP-7抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的BMP-7呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rat BMP-7 concentrations in cell culture supernates, serum, and plasma.
Introduction
The BMPs belong to the TGF- Beta super family, whose members are widely represented throughout the animal kingdom. The BMPs are important regulators of key events in the processes of bone formation during embryo genesis, postnatal growth, remodeling and regeneration of the skeleton. The BMPs function by binding to a receptor complex that is found on all normal cells and is composed of type-I and -II receptors. The primary unit of bone formation is osteoblast, the bone-forming cell. These osteoblast cells respond to physical loading by transducing signals that alter gene expression patterns, and Cbfa (core binding factor), the osteoblast specific transcription factor plays an important role in osteoblast differentiation and function.
BMP activities are modulated through gene expression, protein processing and by interaction with antagonists. The interplay between BMPs and their antagonists such as noggin & chordin governs developmental and cellular processes as diverse as establishment of the embryonic dorsal-ventral axis, induction of neuronal tissue, and formation of joints in the skeletal system and the neurogenesis in the adult brain.
BMP7 is a 431-amino acid polypeptide that includes a secretory signal sequence, (Chr 20), expressed in kidney, bladder and brain. It induces cartilage and bone formation. It may be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. It plays a role in calcium regulation and bone homeostasis.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for BMP-7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP-7 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for BMP-7 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP-7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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