This immunoassay kit allows for the specific measurement of rat Beta-Endorphin concentrations in serum and plasma.
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Beta-Endorphin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Beta-Endorphin present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Beta-Endorphin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Beta-Endorphin bound in the initial step. The color development is stopped and the intensity of the color is measured.
