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预期应用
ELISA法定量测定大鼠血清、血浆或其它相关液体中HMGB1含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中HMGB1水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入HMGB1抗原、生物素化的抗大鼠HMGB1抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的HMGB1呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Rat HMGB1 concentrations in serum and plasma.
Introduction
High Mobility Group Box 1 protein （HMGB1） is a non-histone chomosomal protein that functions in endotoxin lethality. It is released from activated macrophages and is present at elevated levels in the serum of sepsis patients. HMGB1 was also reported to interact with RAGE (receptor for advanced glycation end products) and to influence neuron development.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for HMGB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HMGB1 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for HMGB1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HMGB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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