预期应用
ELISA法定量测定人血清、血浆或其它相关液体中髓磷脂碱性蛋白(MBP)含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中MBP水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入MBP抗原、生物素化的抗人MBP抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的MBP呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human myelin basic protein,MBP concentrations in cell culture supernates, serum, and plasma.
Introduction
Myelin basic protein (MBP) is a protein believed to be important in the process of myelination of nerves in the central nervous system (CNS). Myelin Basic Protein (MBP) is the major constituent of the CNS myelin synthesized by oligodendrocytes and Schwann cells. MBP, PLP (proteolipid proteins) and MOG (myelin oligodendrocyte glycoprotein) are potential primary encephalitogenic target antigens in multiple sclerosis.
MBP was initially sequenced in 1979 after isolation from myelin membranes. Since that time, knockout mice deficient in MBP have been developed which showed decreased amounts of CNS myelination and a progressive disorder characterized by tremors, seizures, and early death. The gene for MBP is on chromosome 18; the protein localizes to the CNS and to various cells of the hematopoietic system.
The pool of MBP in the central nervous system is very diverse, with several splice variants being expressed and a large number of post-translational modifications on the protein, which include phosphorylation, methylation, deamidation and citrullination.
Interest in MBP has centered on its role in demyelinating diseases, particularly multiple sclerosis (MS). Several studies have shown a role for antibodies against MBP in the pathogenesis of MS. Some studies have linked a genetic predisposition to MS to the MBP gene, though a majority have not.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for MBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MBP present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for MBP is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
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