预期应用
ELISA法定量测定人血清、血浆或其它相关液体中TfR含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中TfR水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入TfR抗原、生物素化的抗人TfR抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的TfR呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Human TfR concentrations in serum and plasma.
Introduction
Transferrin Receptor (TfR) is the major mediator of iron uptake by cells. TfR is a transmembrane, disulfide-linked dimer of two identical subunits that binds and internalizes diferric transferrin, thereby delivering iron to the cell cytosol. When a cell needs iron, TfR expression is increased to facilitate iron uptake. Since the major use of iron is for hemoglobin synthesis, about 80% of total TfR is on erythroid progenitor cells.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TfR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TfR present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for TfR is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TfR bound in the initial step. The color development is stopped and the intensity of the color is measured.
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