Von Willebrand Factor因子是一多聚糖蛋白，它与凝血因子VIII以非共价方式结合成复合物存在于血浆中。正常的止血过程中，高分子量多聚体形式的vWF可作为分子桥介导血小板球蛋白IB与内皮下胶原的黏附反应。先天性VWF异常可引起中度至重度出血倾向—此为Von Willebrand病（VWD）。
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中vWF水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入vWF抗原、生物素化的抗vWF抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的vWF呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Human Von Willebrand Factor （vWF）concentrations in cell culture supernates, serum, and plasma.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for VWF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VWF present is bound by the immobilized antibody. An
enzyme-linked monoclonal antibody specific for VWF is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VWF bound in the initial step. The color development is stopped and the intensity of the color is measured.
