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预期应用
ELISA法定量测定人血清、血浆或其它相关液体中 人糖原磷酸化酶同工酶BB（GPBB）含量。
实验原理 本试剂盒应用双抗体夹心酶标免疫分析法测定标本中人糖原磷酸化酶同工酶BB水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入人糖原磷酸化酶同工酶BB、生物素化的抗人糖原磷酸化酶同工酶BB抗体(GPBB)、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的人糖原磷酸化酶同工酶BB呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Human Glycogen phosphorylase BB(GPBB) concentrations in serum and plasma.
Introduction
Human glycogen phosphorylase (GP) b (EC 2.4.1.1) ,particularly its isoenzyme BB (GPBB), has a distinct sensitivity to myocardial oxygen deficiency in cardio-myocytes. There is evidence that GPBB is rapidly released into the circulation in the early phase of AMI as well as after perioperative myocardial infarc-tion following coronary artery bypass grafting .GP in mammals is known to have three major isoen-zymes: BB (brain), MM (muscle), and LL (liver). GPBB is also found in heart muscle ,including human myocardium, where it is, besides the MM subtype, the predominant isoenzyme. The protein sequencededuced from the nucleotide sequence of GPBB cDNA is 83% identical to muscle sequence and 80% identical to liver sequence .
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GPBB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPBB present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for GPBB is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPBB bound in the initial step. The color development is stopped and the intensity of the color is measured.
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