预期应用
ELISA法定量测定人血清、血浆、细胞培养物上清或其它相关液体中内毒素（endotoxin）含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中endotoxin水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入endotoxin、生物素化的抗人endotoxin抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的endotoxin呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Human Endotoxin concentrations in cell culture supernates, serum, and plasma.
Introduction
Endotoxins are potentially toxic, natural compounds found inside pathogens such as bacteria. Classically, an "endotoxin" is a toxin, which unlike an "exotoxin", is not secreted in soluble form by live bacteria, but is a structural component in the bacteria which is released mainly when bacteria are lysed.
Endotoxins are frequent contaminants in plasmid DNA prepared from bacteria, and must be removed from the DNA to avoid unwanted inflammatory responses prior to in vivo applications such as gene therapy.In pharmaceutical production, it is necessary to remove all traces of endotoxin from drug product containers as even small amounts of endotoxin will cause illness, but not disease, in humans.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Endotoxin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Endotoxin present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for Endotoxin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Endotoxin bound in the initial step. The color development is stopped and the intensity of the color is measured.
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