预期应用
ELISA法定量测定兔血清、血浆或其它相关液体中 P物质含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中P物质水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入P物质抗原、生物素化的抗兔P物质抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的P物质呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rabbit Substance P concentrations in serum and plasma.
Introduction
Substance P is a bioactive 12-amino acid peptide (Arg- Pro- Lys- Pro- Gln- Gln- Phe- Phe- Gly- Leu- Met-amide) first isolated in 1931 from brain and intestine. The peptide is involved in many physiological processes including pain modulation, smooth muscle contraction, blood pressure control, kidney function and water homeostasis. Substance P is widely distributed in numerous tissues and body fluids including the central and peripheral nervous system, gastrointestinal tract, respiratory tract, visual system and circulatory system.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for Substance P has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Substance P present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Substance P is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Substance P bound in the initial step. The color development is stopped and the intensity of the color is measured.
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