预期应用
ELISA法定量测定大鼠血清、血浆、细胞培养物上清或其它相关液体中多巴胺（dopamine）含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中多巴胺水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入多巴胺、生物素化的抗大鼠多巴胺抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的多巴胺呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rat Dopamine concentrations in cell culture supernates, serum, and plasma.
Introduction
Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport.
Dopamine is a hormone and neurotransmitter occurring in a wide variety of animals, including both vertebrates and invertebrates. Chemically, it is a phenethylamine.Dopamine is produced in several areas of the brain, including the substantia nigra. Dopamine is also a neurohormone released by the hypothalamus. Its main function as a hormone is to inhibit the release of prolactin from the anterior lobe of the pituitary. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4).
Dopamine can be supplied as a medication that acts on the sympathetic nervous system, producing effects such as increased heart rate and blood pressure. However, since dopamine cannot cross the blood-brain barrier, dopamine given as a drug does not directly affect the central nervous system. To increase the amount of dopamine in the brains of patients with diseases such as Parkinson s disease and Dopa-Responsive Dystonia, L-DOPA (levodopa), which is the precursor of dopamine, can be given because it can cross the blood-brain barrier.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Dopamine has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Dopamine present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for Dopamine is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Dopamine bound in the initial step. The color development is stopped and the intensity of the color is measured.
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