Intended use
This immunoassay kit allows for the specific measurement of human complement C5b-9 concentrations in serum and plasma.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for complement C5b-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any complement C5b-9 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for complement C5b-9 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of complement C5b-9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
