本试剂盒应用双抗体夹心酶标免疫分析法测定标本中Lptn水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入Lptn抗原、生物素化的抗大鼠Lptn抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Lptn呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Rat lymphotactin (Lptn) concentrations in cell culture supernates, serum and plasma.
Introduction
Rat lymphotactin (Lptn) belong to the C or γ subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Rat lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells.
Chemokines are proteins that have important physiological and pathophysiological roles in a wide range of acute and chronic inflammatory processes. Chemokines exert their biological effects by binding to cell surface receptors. Their sequences are similar and are characterised by a 4-cysteine motif: the family can be divided according to whether the first 2 Cys residues are adjacent (the C-C family), separated by an intervening residue (the C-x-C family), have only one of the first two Cys residues (C chemokines) , or contain both cysteines , separated by three intervening residues (C-x3-C chemokines).
Lymphotactin is the only known member of the C chemokine family. It has closest similarity to the C-C chemokines but contains only the second and fourth of the conserved cysteine residues. The chemokine is produced by certain subsets of T cells and natural killer cells and is also chemotactic for these cell types.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Lptn has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Lptn present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Lptn is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Lptn bound in the initial step. The color development is stopped and the intensity of the color is measured.
