预期应用
ELISA法定量测定犬血清、血浆、细胞培养物上清或其它相关液体中肌红蛋白含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中肌红蛋白水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入肌红蛋白抗原、生物素化的抗犬肌红蛋白抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的肌红蛋白呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of canine Myoglobin concentrations in serum and plasma.
Introduction
Myoglobin is a single polypeptide chain of 153 amino acids and molecular mass of approximately 17,500 Daltons. It contains one heme prosthetic group per molecule and is found only in striated skeletal and cardiac muscle. During the past several years the usefulness of monitoring serum myoglobin in the detection of early AMI has been documented in several studies. It has also been documented in number of studies that myoglobin has far superior clinical sensitivity than CK-MB in early detection of AMI in emergency room patients.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for Myoglobin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Myoglobin present is bound by the immobilized antibody. An
enzyme-linked monoclonal antibody specific for Myoglobin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells
and color develops in proportion to the amount of Myoglobin bound in the initial step. The color
development is stopped and the intensity of the color is measured.
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