预期应用
ELISA法定量测定大鼠血清、血浆、尿液或其它相关液体中FFA含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中FFA水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入FFA抗原、生物素化的抗大鼠FFA抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的FFA呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Rat FFA concentrations in serum and plasma.
Introduction
In chemistry, especially biochemistry, a fatty acid is a carboxylic acid often with a long unbranched aliphatic tail (chain), which is either saturated or unsaturated. Carboxylic acids as short as butyric acid (4 carbon atoms) are considered to be fatty acids, while fatty acids derived from natural fats and oils may be assumed to have at least 8 carbon atoms, e.g. caprylic acid (octanoic acid). Most of the natural fatty acids have an even number of carbon atoms, because their biosynthesis involves acetyl-CoA, a coenzyme carrying a two-carbon-atom group.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for FFA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FFA present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for FFA is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FFA bound in the initial step. The color development is stopped and the intensity of the color is measured.
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