预期应用
ELISA法定量测定大鼠血浆或其它相关液体中GP Ⅱb/Ⅲa含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中GP Ⅱb/Ⅲa水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入GP Ⅱb/Ⅲa抗原、生物素化的抗大鼠GP Ⅱb/Ⅲa抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的GP Ⅱb/Ⅲa呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rat GP Ⅱb/Ⅲa concentrations in cell culture supernates, serum, and plasma.
Introduction
Platelet glycoprotein Ⅱb/Ⅲa (GP Ⅱb/Ⅲa) is the receptor of fibrinogen. Platelet cross-linking with fibrinogen through GPⅡb/Ⅲa is the process of thrombosis.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for GP Ⅱb/Ⅲa has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GP Ⅱb/Ⅲa present is bound by the immobilized antibody. An
enzyme-linked monoclonal antibody specific for GP Ⅱb/Ⅲa is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells
and color develops in proportion to the amount of GP Ⅱb/Ⅲa bound in the initial step. The color
development is stopped and the intensity of the color is measured.
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