预期应用
ELISA法定量测定大鼠血清、血浆或其它相关液体中NF-κ/B P65含量。
产品介绍
NF-κB P65是一种重要的转录因子，其激活参与炎症、细胞增殖、细胞凋亡等基因的调节，影响着细胞的凋亡，同时影响着肿瘤细胞对细胞毒性药物及离子辐射的敏感性。ras基因诱导的致癌突变作用需NF-κB的活化，表明NF-κB在致癌发生方面可能起一定作用；NF-KBp65可以保护细胞免受肿瘤坏死因子以及电离辐射等引起的凋亡作用，而抑制NFkB的表达可以增加TNF等引起的细胞凋亡，以及增加化疗及放疗对肿瘤细胞的敏感性。
NF-κB由两类亚基形成同源或异源二聚体。一类亚基包括p65(也称RelA)、RelB和C-Rel；另一类亚基包括p50和p52。最常见的NF-κB亚基组成形式为p65/p50或p65/p65。bNF-κB是一种常见的转录因子，可以被炎症因子、生长因子或趋化因子等激活。
Intended use
This immunoassay kit allows for the specific measurement of Rat NF-κB/P65 concentrations in cell culture supernates, serum and plasma.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for NF-κB/P65 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NF-κB/P65 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for NF-κB/P65 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NF-κB/P65 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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