本试剂盒应用双抗体夹心酶标免疫分析法测定标本中对环磷酰胺水平。用纯化的抗体包被微孔板，制成固相抗体，往包被抗体的微孔中依次加入对环磷酰胺、生物素化的抗人对环磷酰胺抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的对环磷酰胺呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Human Cyclophosphamide,CTX concentrations in cell culture supernates, urine ,serum, and plasma.
Introduction
Cyclophosphamide (the generic name for Cytoxan, Neosar), also known as cytophosphane, is a nitrogen mustard alkylating agent, used to treat various types of cancer and some autoimmune disorders. It is a "prodrug"; it is converted in the liver to active forms that have chemotherapeutic activity.The main use of cyclophosphamide is together with other chemotherapy agents in the treatment of lymphomas, some forms of leukemia and some solid tumors. It is a chemotherapy drug that works by slowing or stopping cell growth. It also works by decreasing the immune systems response to various diseases.
In addition, its use is becoming more common in autoimmune diseases where disease-modifying antirheumatic drugs (DMARDs) have been ineffective. Systemic lupus erythematosus (SLE) with severe lupus nephritis, for example, may respond to pulsed cyclophosphamide. However in 2005, standard treatment for lupus nephritis changed to MMF from cyclophosphamide.
It is also used to treat Minimal Change Disease and rheumatoid arthritis. It is still used for Wegeners granulomatosis. The trade name is Endoxan.
In order to work, cyclophosphamide first is converted by the liver into two chemicals, acrolein and phosphoramide. Acrolein and phosphoramide are the active compounds, and they slow the growth of cancer cells by interfering with the actions of deoxyribonucleic acid (DNA) within the cancerous cells. It is, therefore, referred to as a cytotoxic drug. Unfortunately, normal cells also are affected, and this results in serious side effects. Cytoxan also suppresses the immune system and is also referred to as immunosuppressive.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Cyclophosphamide has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Cyclophosphamide present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for Cyclophosphamide is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Cyclophosphamide bound in the initial step. The color development is stopped and the intensity of the color is measured.
