预期应用
ELISA法定量测定人血清、血浆、细胞培养物上清或其它相关液体中CK20含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中CK20水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入CK20抗原、生物素化的抗人CK20抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的CK20呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human CK20 concentrations in cell culture supernates, serum, and plasma.
Introduction
Cytokeratin (CK) 20 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract. Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium. CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for CK20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CK20 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for CK20 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CK20 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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