本试剂盒应用双抗体夹心酶标免疫分析法测定标本中胸腺肽水平。用纯化的抗体包被微孔板，制成固相抗体，往包被抗体的微孔中依次加入胸腺肽、生物素化的抗人胸腺肽抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的胸腺肽呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Human Thymosin concentrations in cell culture supernates, serum, and plasma.
Introduction
Thymosin is a hormone secreted from the thymus. Its primary function is to stimulate the production of T cells, which are an important part of the immune system.Thymosin also assists in the development of B cells to plasma cells to produce antibodies.
The predominant form of thymosin, thymosin β4, is a member of a highly conserved family of actin monomer-sequestering proteins. β-thymosins are the primary regulators of unpolymerized actin, and are essential for maintaining the small cytoplasmic pool of free G-actin monomers required for rapid filament elongation and allowing for the flux of monomers between the thymosin-bound pool and F-actin .
Thymosin β4 sequesters actin, holding it in a form that is unable to polymerize. Due to its profusion in the cytosol and its ability to bind ATP G-actin but not F-actin, thymosin β4 is regarded as the principal actin-sequestering protein. Tβ4 binds ATP G- monomeric actin in a 1:1 complex where G-actin cannot polymerize.
Increase in cytosolic concentrations of thymosin β4 increases the concentration of sequestered actin subunits and correspondingly decreases F-actin due to actin filaments being in equilibrium with actin monomers.Furthermore, the inhibitory thymosin which sequesters the actin competes for monomers with profilin.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Thymosin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Thymosin present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for Thymosin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Thymosin bound in the initial step. The color development is stopped and the intensity of the color is measured.
