预期应用
ELISA法定量测定人血清、血浆或其它相关液体中组织多肽特异性抗原(TPS)含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中TPS水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入TPS、生物素化的抗人TPS抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的TPS呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human tissue polypeptide specific antigen,TPS concentrations in cell culture supernates, serum, and plasma.
Introduction
Tissue polypeptide antigen (TPA) is a heterogene- ous combination of molecules of molecular weight between 20-45 kDa. It was first defined as a tumor associated antigen in 1957 by Bjorklund. Immunologically TPA is defined as an aggregate of nonepidermal cytokines 8, 18, 19 . TPS was characterized by the development of a monoclonal anti-body against subgroups of TPA. Further studies have proved the similarity between M3 epitope of TPA and the second part of cytokine 18 .TPS is the specific M3 epitope of TPA and also known as cytokine 18. It has been used as a prognostic parameter for some neoplasms since it was isolated in the serum or urine of some cancer patients.TPS is most frequently elevated in malignant tissues (gynecologic, prostate, GIS, lung, etc). Serum TPS levels not only reflect tumor mass but also tumor activity and are related with proliferation more than necrosis. Furthermore, elevated TPS levels can also be detected in some benign events such as liver failure, renal failure, gestati-on, generalized infection, and diabetes mellitus(DM) .
TPS levels in cystic fluid, peritoneal fluid, and blood could be used as a marker for the management of ovarian mass.The value of serum tissue polypeptide specific antigen (TPS) as a complement to CA 19-9 in the detection of pancreatic carcinoma was determined prospectively. TPS and CA 19-9 levels obtained at the time of diagnosis in patients suspected of having chronic pancreatitis or pancreatic carcinoma were evaluated in receiver operating characteristic (ROC) curve analysis.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for TPS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPS present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for TPS is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPS bound in the initial step. The color development is stopped and the intensity of the color is measured.
| 
