预期应用
ELISA法定量测定大鼠血清、血浆、细胞培养物上清或其它相关液体中Smad1含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中Smad1水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入Smad1抗原、生物素化的抗大鼠Smad1抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Smad1呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rat Smad1 concentrations in cell culture supernates, serum, and plasma.
Introduction
Bone morphogenetic proteins (BMPs) are members of the TGF-b family that regulates cell proliferation, apoptosis, and differentiation (1–2). Smad1 functions downstream of BMP receptors and undergoes serine phosphorylation in response to receptor activation. Phosphorylation of Smad1 involves serine 463 and 465, in the carboxy-terminal motif SSXS. As a direct physiological substrate of BMP receptors, Smad1 provides a link between receptor serine/threonine kinases and the nucleus (3).
References:
Hogan, B.L.M. (1996) Genes Dev. 10, 1580-1594.
Hoodless, P.A. et al. (1996) Cell 85, 489-500.
Marcus, K. et al. (1997) Genes Dev. 11, 984-995.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for Smad1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Smad1 present is bound by the immobilized antibody. An
enzyme-linked monoclonal antibody specific for Smad1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells
and color develops in proportion to the amount of Smad1 bound in the initial step. The color
development is stopped and the intensity of the color is measured.
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