预期应用
ELISA法定量测定人血清、血浆或其它相关液体中Ig-j 含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中Ig-j 水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入Ig-j 抗原、生物素化的抗人Ig-j 抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Ig-j 呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human Ig-j concentrations in serum and plasma.
Introduction
Joining (J) chain is a component of polymeric, but not monomeric, immunoglobulin (Ig) molecules and may play a role in their polymerization and transport across epithelial cells. The J chain is critical in the structure of SIgA because it is required for efficient polymerization of IgA and for the affinity of such polymers to the secretory component (SC)/polymeric (p)IgR.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Ig-j has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Ig-j present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Ig-j is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Ig-j bound in the initial step. The color development is stopped and the intensity of the color is measured.
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