本试剂盒应用双抗体夹心酶标免疫分析法测定标本中KLK 8水平。用纯化的抗体包被微孔板，制成固相抗体，往包被抗体的微孔中依次加入KLK 8抗原、生物素化的抗人KLK 8抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的KLK 8呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human Kallikrein 8,KLK 8 concentrations in cell culture supernates, serum, and plasma.
Introduction
Kallikrein 8 (hK8), also known as Neuropsin, Brain Serine Protease 1 (BSP1), tumor associated differentially expressed gene 14 (TADG14), PRSS19, HNP and Ovasin is a trypsin like serine proteinase. The name neuopsin is intended to indicate a neuronal trypsin like proteinase, but a literature alias for a form of opsin 5, also called neuopsin, confuses things a bit. Originally described from mouse brain, KLK8 was implicated in neural plasticity and learning. Kallikrein 8 is thought to be involved in neurite outgrowth and fasciculation during nervous system development. Kallikrein 8 is induced in neurite injury, and Kallikrein 8 has been shown to be elevated in the hippocampus on Alzheimer’s patients’ brains. Kallikrein 8 is thought to regulate the early phase of long-term potentiation (LTP), possibly by cleaving fibronectin and other substrates.Kallikrein 8 is also found in the uterus, where it is hormonally regulated. Cervical cancer cell lines express high levels of Kallikrein 8, and it may be useful as a tumor marker. Kallikrein 8 is not limited to the brain and uterus, as many tissues and cell lines make the protein. Endogenous inhibitors include serine proteinase inhibitor 3, protein C inhibitor and 1 proteinase inhibitor, although Kallikrein 8 can be found complexed to a number of different proteinase inhibitors. A number of different splice variants and partial sequences have been reported for Kallikrein 8, the longest encodes a protein of 305 amino acids with a predicted mass of 32.97 kDa and a pI of 8.59. The 260 amino acid form has a predicted mass of 28 kDa and pI of 8.18, the 164, 134 and 119 amino acid versions 17.9, 14.7 and 12.7 kDa respectively, but it is unclear if these proteins are produced.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for Kallikrein 8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Kallikrein 8 present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for Kallikrein 8 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Kallikrein 8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
