预期应用
ELISA法定量测定人血清、血浆或其它相关液体中Hb-AGEs含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中Hb-AGEs水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入Hb-AGEs抗原、生物素化的抗人Hb-AGEs抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Hb-AGEs呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human Hb-AGEs concentrations in serum and plasma.
Introduction
Advanced glycosylation end products (AGEs) form spontaneously from glucose-derived Amadori products and accumulate on long-lived tissue proteins. AGEs have been implicated in the pathogenesis of several of the complications of aging and diabetes, including atherosclerosis and renal disease. With the use of recently developed AGE-specific antibodies, an AGE-modified form of human hemoglobin has been identified.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for Hb-AGEs has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Hb-AGEs present is bound by the immobilized antibody. An
enzyme-linked monoclonal antibody specific for Hb-AGEs is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells
and color develops in proportion to the amount of Hb-AGEs bound in the initial step. The color
development is stopped and the intensity of the color is measured.
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