预期应用
ELISA法定量测定小鼠血清、血浆或其它相关液体中ADH含量。
实验原理 本试剂盒应用双抗体夹心酶标免疫分析法测定标本中Testosterone水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入Testosterone抗原、生物素化的抗小鼠Testosterone抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Testosterone呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of mouse ADH concentrations in serum and plasma.
Introduction
Arginine vasopressin (AVP), also known as argipressin, antidiuretic hormone (ADH), or simply as vasopressin, is a nine amino acid peptide hormone that plays a primary role in the regulation of renal water excretion and a secondary role in the regulation of cardiovascular function in mammals.1,2,3 AVP acts on epithelial cells of the urinary tract to augment the reabsorption of water, resulting in concentration of urine and dilution of blood serum. It also stimulates the constriction of capillaries and arterioles, elevating blood pressure.4 Normal levels of AVP in serum are 0.4-5.2 pg/ml.5,6 Plasma levels of AVP are elevated in patients with major and also anxious-retarded depression, as well as in patients with congestive heart failure.5,7,8,9
1. Brownstein, M.J., Russell, J.T., Gainer, H. Synthesis, transport, and release of posterior pituitary hormones. Science 207 373-378 (1980).
2. Robertson, G.L., Berl, T. Pathophysiology of water metabolism. 4th, 677-736 (1991).
3. St-Louis, J., Schiffrin, E.L. Biological action and binding sites for vasopressin on the mesenteric artery from normal and sodium-depleted rats. Life Sci 35 1489-1495 (1984).
4. Thionnier, M. Signal transduction of V1-vascular vasopressin receptors. Regul Pept 38 1-11 (1992).
5. van Londen, L., Goekoop, J.G., van Kempen, G.M., et al. Plasma levels of arginine vasopressin elevated in patients with major depression. Neuropsychoparmacology 17 284-292 (1997).
6. Robertson, G.L., Mahr, E.A., Athar, S., et al. Development and clinical application of a new method for the radioimmunoassay of arginine vasopressin in human plasma. J Clin Invest 52 2340-2352 (1973).
7. de Winter, R.F.P., van Hemert, A.M., DeRijk, R.H., et al. Anxious-retarded depression: Relation with plasma vasopressin and cortisol. Neuropsychoparmacology 23 140-147 (2003).
8. Creager, M.A., Faxon, D.P., Cutler, S.S., et al. Contribution of vasopressin to vasoconstriction in patients with congestive heart failure: Comparison with the renin-angiotensin system and the sympathetic nervous system. J Am Coll Cardiol 7 758-765 (1986).
9. Price, J.F., Towbin, J.A., Denfield, S.W., et al. Arginine vasopressin levels are elevated and correlate with functional status in infants and children with congestive heart failure. Circulation 109 2550-2553 (2004)
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for ADH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADH present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for ADH is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADH bound in the initial step. The color development is stopped and the intensity of the color is measured.
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